Biotech 2: Recombinant DNA Lab*Z (BIOL 471)

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• Credit Hours: Min: 3, Max: 3

Practical training in recombinant DNA techniques. The term project includes tissue DNA isolation, restrictions, electrophoresis, and Southern transfer; plasmid (ultracentrifugal) DNA isolation; and restriction; isolation of cloned gamma crystallin gene; non-radioactive labelling of cloned insert; and hybridization to Southern transfer. RFLP analysis of genetic defect in blind rats. This course meets General Education requirements: Scientific Understanding, Lab. Prerequisites Corequisite: BIOL 470