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BIOL 471

Min credits: 3

Recombinant DNA Laboratory

Max credits: 3
Practical training in recombinant DNA techniques. The term project includes tissue DNA isolation, restrictions, electrophoresis, and Southern transfer; plasmid (ultracentrifugal) DNA isolation; and restriction; isolation of cloned gamma crystallin gene; non-radioactive labelling of cloned insert; and hybridization to Southern transfer. RFLP analysis of genetic defect in blind rats. This course meets General Education requirements: Scientific Understanding, Lab.

Requires:

BIOL 375 and CHEMM 333

 

 

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