Proteins (BIOL 472)

• College: College of Arts and Sciences
• Department: Biology
• Credit Hours: 3
• This course has a lecture with 3 hours.

The theory, molecular mechanisms, and practical aspects of the major techniques used in protein purification. Emphasis is placed on data interpretation and manipulation, trouble-shooting, and prediction of the impact of various errors on the data, for each technique. Included are: enzyme kinetics; buffer design; cell disruption; differential solubility; ion exchange, gel permeation, and affinity chromatography; ultracentrifugation; chromatofocusing; radioisotope use; PAGE; and IEF. This course meets General Education requirements: Scientific Understanding. Pre-Requisites: BIOL 122 and CHEM 322 with a grade of C- or better. Typically Offered Fall Only, Odd Years