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BIOL 472
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Min credits: |
3 |
Proteins*Z
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Max credits: |
3 |
| The theory, molecular mechanisms, and practical aspects of the major techniques used in protein purification. Emphasis is placed on data interpretation and manipulation, trouble-shooting, and prediction of the impact of various errors on the data, for each technique. Included are: enzyme kinetics; buffer design; cell disruption; differential solubility; ion exchange, gel permeation, and affinity chromatography; ultracentrifugation; chromatofocusing; radioisotope use; PAGE; and IEF. This course meets General Education requirements: Scientific Understanding. |
Requires:
CHEM 364 and BIOL 122
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